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Biotin anti-human CCL22 Antibody

Pricing & Availability
Clone
A15083D (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1)
Isotype
Mouse IgG2a, κ
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Product Citations
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A15083D_BIOTIN_-CCL22_Antibody_050323
Direct ELISA showing biotin anti-human CCL22 (MDC) (clone A15083D) bound to plate-immobilized recombinant human CCL22 (MDC) protein (Cat. Nos. 584902 and 584904). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A15083D_BIOTIN_-CCL22_Antibody_050323
    Direct ELISA showing biotin anti-human CCL22 (MDC) (clone A15083D) bound to plate-immobilized recombinant human CCL22 (MDC) protein (Cat. Nos. 584902 and 584904). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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694405 50 µg $235
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Description

CCL22 is thymus-specific chemokine engaged in the recruitment of T cells. CCL22 is associated with different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CCL22
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Application Notes
  • Clones A15083A and A15083D do not block each other
  • Clone A15083A does not block the reference clone T51-719 (BD Bioscience)
  • Clones A15083A and A15083D showed good staining in both Cyto-Fast™ Fix/Perm Buffer Set and True-Phos™ Perm Buffer.
Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A152083D) could function as a detection antibody when paired with purified anti-human CCL22 (MDC) antibody (Cat. No. 611952) (clone A15083A) as the capture antibody and using Recombinant Human CCL22 (MDC) (carrier-free) protein (Cat. Nos. 584902 and 584904) as the assay standard.

 

RRID
AB_3097162 (BioLegend Cat. No. 694405)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, NK cells, IFN-γ stimulated human keratinocytes, thymic epithelial cells, and intestinal epithelial cells

Function
CCL22 induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFN-γ).
Interaction
Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor
CCR4
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Godiska R, et al. 1997. J Exp Med. 185:1595-604.
  2. Imai T, et al. 1998. J Biol Chem. 273:16551-4.
  3. Yamashita U and Kuroda E. 2002. Crit Rev Immunol. 22:105-14.
  4. Horikawa T, et al. 2002. Int Immunol. 14:767-73.
  5. Mailloux AW and Young MR. 2009. J Immunol. 182:2753-65.
  6. Dogan RN, et al. 2011. J Leukoc Biol. 89:93-104.
  7. Osborn O, et al. 2011. Cytokine. 53:311-9.
Gene ID
6367 View all products for this Gene ID
UniProt
View information about CCL22 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 05/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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