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- Clone
- Poly5210 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HC14, MCP-2
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 521003 | 50 µg | $375 | ||||
CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells that are stimulated with IL-1β. CCL8 (MCP-2) has a structure and function that is similar with both MCP-1 and MCP-3. It is often co-induced with those chemokines in mononuclear cells. IFNγ is a strong inducer of CCL8 (MCP-2) in monocytes and fibroblasts. CCL8 (MCP-2) in monocytes and fibroblasts and can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemo attract monocytes, but it binds to the CCR2 receptor and induces its internalization. As a result, CCL8 (MCP-2) inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 (MCP-2) is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of the Th2 homing, which drives IL-5 mediated chronic allergic inflammation. In addition, CCL8 (MCP-2) binds the CCR5 receptor with a high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 (MCP-2) is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human CCL8.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25 – 1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 250 to 3.9 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Detection: The biotinylated Poly5210 antibody is useful as the detection antibody for a sandwich ELISA assay, when used in conjunction with the purified L3715B6 antibody as the capture antibody and recombinant human CCL8 (MCP-2) as the standard.
Note: For testing human CCL8 (MCP-2) in serum, plasma, or supernatant, BioLegend’s LEGEND MAX™ Human CCL8 (MCP-2) ELISA Kit is specially developed and recommended. - RRID
-
AB_2565224 (BioLegend Cat. No. 521003)
Antigen Details
- Structure
- Chemokine.
- Distribution
- Fibroblasts, endothelial cells, monocytes, and macrophages.
- Function
- CCL8 (MCP-2) chemo attracts monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils. CCL8 (MCP-2) is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 (MCP-2) by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
- Interaction
- Monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils.
- Ligand/Receptor
- CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
- Cell Type
- Fibroblasts, Endothelial cells, Monocytes, Macrophages
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.
2. Proost P, et al. 1998. J. Immunol. 160:4034.
3. Dean RA, et al. 2008. Blood 112:3455.
4. Hori T, et al. 2008. Blood 111:4403.
5. Rom S, et al. 2010. FASEB J. 24:2292.
6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
7. Islam SA, et al. 2011. Nat. Immunol. 12:167. - Regulation
- Monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils.
- Gene ID
- 10635 View all products for this Gene ID
- UniProt
- View information about CCL8 on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All CCL8 (MCP-2) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human CCL8 (MCP-2) | Poly5210 | ELISA Detection |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human CCL8 (MCP-2)
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