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- Clone
- BL25372 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B, Interferon Alpha 2
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Coating Buffer B, M4509E12 at 4 µg/mL as capture antibody, Blocked Assay Diluent A (ADA) 1 hr, lyophilized standard 2 hr in Assay Buffer G 50:50 with Matrix A run 12.5 – 800 pg/mL, BL25372 (MT-2) at 0.1 µg/mL 1 hr for detection in ADA, 0.8 µg/mL Avidin HRP in ADA 30 min, TMB F 15 min (in house 76335).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 537204 | 100 µg | $265 | ||||
Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α; therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.05 - 0.4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-α2 recombinant protein ranging from 12.5 to 800 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The biotinylated BL25372 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M4509E12 antibody (Cat. No. 537102) as the capture antibody and Recombinant Human IFN-a2 (ELISA Std.) (Cat. No. 592709)
- RRID
-
AB_2783347 (BioLegend Cat. No. 537204)
Antigen Details
- Distribution
-
IFN-α is ubiquitously expressed.
- Function
- IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
- Interaction
- A variety of cells express the IFNα receptor (IFNAR).
- Ligand/Receptor
- IFNAR complex has two components, IFNAR1 and IFNAR2.
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Bose A and Baral R. 2007. Immunol Lett. 108:68.
- Zhao W, et al. 2008. J Immunol. 180:5483.
- Badiger R, et al. 2012. Plos One. 7:e46779.
- Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485.
- Becker-Merok A, et al. 2013. Lupus. 22:155.
- Mukherjee KK, et al. 2012. Indian J Med Res. 136:54.
- Gene ID
- 3440 View all products for this Gene ID
- UniProt
- View information about IFN-alpha2 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All IFN-α2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IFN-α2 | BL25372 | ELISA Detection |
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Biotin anti-human IFN-α2
Coating Buffer B, M4509E12 at 4 µg/mL as capture antibody, B...
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