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Biotin anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B_A2F10_Biotin_120209
C57BL/6 bone marrow cells stained with CD45R/B220 + CD11b APC and A2F10 biotin, followed by Sav-PE
  • B_A2F10_Biotin_120209
    C57BL/6 bone marrow cells stained with CD45R/B220 + CD11b APC and A2F10 biotin, followed by Sav-PE
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135307 50 µg $83
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135308 500 µg $288
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Feng J, et al. 2022. Immunity. 55:405. PubMed
  2. Andersson S, et al. 2012. PLoS One. 7:e47668. PubMed
  3. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  4. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  5. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  6. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  7. Chavez JS, et al. 2022. Cells. 11:. PubMed
  8. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  9. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  10. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  11. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_1953266 (BioLegend Cat. No. 135307)
AB_1953266 (BioLegend Cat. No. 135308)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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