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Biotin anti-mouse CD19 Antibody

Pricing & Availability
Clone
MB19-1 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Mouse IgA, κ
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Product Citations
publications
mb19-1
C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE
  • mb19-1
    C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE
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101504 500 µg $270
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Description

CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
mouse CD19+ pre-B cell line 300.19
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Sato S, et al. 1997. J. Immunol. 158:4662. (Costim)
  2. Sato S, et al. 1997. P. Natl. Acad. Sci. USA 94:13158. (Costim)
  3. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (IP)
  4. Sato S, et al. 1996. J. Immunol. 157:4371.
  5. Stolk M, et al. 2006. J. Leukoc. Biol. doi:10.1189/jlb.1205739. (FC)
  6. Shime H, et al. 2012. PNAS. 109:2066. PubMed.
Product Citations
  1. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  2. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  3. Becker M, et al. 2017. Proc Natl Acad Sci U S A. 114:5231. PubMed
  4. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  5. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  6. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  7. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  8. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  9. Shime H, et al. 2012. Proc Natl Acad Sci U S A. 109:2066. PubMed
RRID
AB_312823 (BioLegend Cat. No. 101504)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF,et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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