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Biotin anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_Biotin_090507
C57BL/6 mouse splenocytes stained with biotinylated 30-F11, followed by Sav-PE
  • 30-F11_Biotin_090507
    C57BL/6 mouse splenocytes stained with biotinylated 30-F11, followed by Sav-PE
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103103 50 µg $54
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103104 500 µg $242
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  2. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
  3. Gbyli R, et al. 2022. Leukemia. 36:1313. PubMed
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  5. Dell'Aringa M, et al. 2018. Mucosal Immunol. 1.207638889. PubMed
  6. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  7. Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed
  8. Papadopoulou D, et al. 2023. JCI Insight. 8:. PubMed
  9. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
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  12. Fujimori S, et al. 2022. Elife. 11:. PubMed
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  14. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  15. Kaminitz A, et al. 2013. Int Immunol. 25:485. PubMed
  16. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  17. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  18. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  19. Muhsin-Sharafaldine M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.138. PubMed
  20. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
  21. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  22. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  23. Ibrahim AM, et al. 2021. Breast Cancer Res. 23:90. PubMed
  24. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
  25. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  26. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  27. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  28. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
  29. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  30. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
  31. Howard E, et al. 2020. J Allergy Clin Immunol. . PubMed
  32. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  33. Nevius E, et al. 2015. J Exp Med. 212: 1931 - 1946. PubMed
  34. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  35. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
  36. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
  37. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  38. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  39. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
  40. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
  41. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  42. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  43. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  44. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  45. Barnes SE, et al. 2021. J Neuroinflammation. 18:199. PubMed
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  47. Pual VM, et al. 2019. PLOS Biology. 17(10):e3000492. PubMed
  48. Budai Z, et al. 2021. Cells. 10:. PubMed
  49. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  50. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
  51. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  52. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  53. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  54. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  55. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
  56. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  57. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
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  60. Seong J, et al. 2018. Development. 145:14. PubMed
RRID
AB_312969 (BioLegend Cat. No. 103103)
AB_312969 (BioLegend Cat. No. 103104)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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