Loading...

Biotin anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
53-6dot7_Biotin_041106
C57BL/6 mouse splenocytes were stained with biotinylated CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
  • 53-6dot7_Biotin_041106
    C57BL/6 mouse splenocytes were stained with biotinylated CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability Save
100703 50 µg $42
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100704 500 µg $88
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  2. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  3. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  4. Marié IJ, et al. 2022. Bio Protoc. 12:e4438. PubMed
  5. Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed
  6. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  7. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  8. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  9. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  10. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  11. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  12. McGee MC, et al. 2022. Bio Protoc. 12:. PubMed
  13. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  14. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  15. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  16. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  17. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  18. Mendes A, et al. 2021. Life Sci Alliance. 4:00. PubMed
  19. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  20. Elsner R, et al. 2015. PLoS Pathog. 10: 1004976. PubMed
  21. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  22. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  23. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  24. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  25. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  26. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  27. Zhou L, et al. 2021. Cell Mol Immunol. 18:698. PubMed
  28. Donley D, et al. 2016. PLoS One. 11: 0162404. PubMed
  29. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  30. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  31. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  32. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  33. Iturri L, et al. 2021. Immunity. . PubMed
  34. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  35. Hutter K, et al. 2020. Molecular Cell. 78(5):876-889.e6. PubMed
  36. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  37. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  38. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  39. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  40. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  41. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  42. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  43. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  44. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  45. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  46. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  47. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  48. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  49. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  50. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  51. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  52. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  53. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  54. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  55. Köchl R, et al. 2020. Elife. 9:00. PubMed
  56. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  57. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  58. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  59. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  60. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  61. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  62. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  63. Dong S, et al. 2021. Nature. 591:117. PubMed
  64. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  65. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  66. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  67. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  68. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  69. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  70. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  71. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  72. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  73. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  74. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  75. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  76. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  77. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  78. Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed
  79. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  80. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  81. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  82. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  83. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  84. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  85. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  86. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  87. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  88. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  89. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  90. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  91. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  92. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  93. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  94. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
  95. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  96. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  97. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  98. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  99. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  100. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  101. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  102. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  103. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  104. Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
  105. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  106. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  107. Young K, et al. 2021. Cell Stem Cell. . PubMed
  108. Davidson M, et al. 2013. PLoS One. 8:76258. PubMed
  109. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  110. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_312742 (BioLegend Cat. No. 100703)
AB_312742 (BioLegend Cat. No. 100704)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Go To Top Version: 2    Revision Date: 01/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account