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Biotin anti-mouse IgG2b Antibody

Pricing & Availability
Clone
RMG2b-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Rat IgG1, κ
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
406703 50 µg $83
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406704 500 µg $206
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Description

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
ELISA
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Heyburn L, et al. 2016. J Neurochem. 610:139. PubMed
  2. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  3. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  4. Sellars M, et al. 2009. J Exp Med. 206:1073. PubMed
  5. Esposito L, et al. 2014. J Immunol. 193:889. PubMed
  6. Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed
  7. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  8. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
RRID
AB_315067 (BioLegend Cat. No. 406703)
AB_315067 (BioLegend Cat. No. 406704)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 02/12/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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