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Biotin anti-mouse IL-17F Antibody

Pricing & Availability
Clone
8F5.1A9 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17F, ML-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
IL17AF_Mouse_ELISA_011614
  • IL17AF_Mouse_ELISA_011614
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517105 50 µg $176
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Description

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Overexpression of the IL-17F gene in the airways of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo.  P38 MAPK, ERK1/2, Act1 (NF-kB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-17F-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1.0-4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 500 to 7.8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated 8F5.1A9 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10.1 (Cat. No. 506901 or 506906) antibody as the capture antibody and recombinant mouse IL-17A/F (Cat. No. 580809) as the standard.

Flow Cytometry: The fluorochrome-labeled 8F5.1A9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17F-producing cells within mixed cell populations.

Note: For testing mouse IL-17F in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 436107 & 436108) are specially developed and recommended.

RRID
AB_2562987 (BioLegend Cat. No. 517105)

Antigen Details

Structure
Forms a 37 kD homodimer or heterodimer with IL-17A, a IL-17 family member.
Distribution
Th17 cells, mast cells, basophils, and epithelial cells.
Function
An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor
IL-17R (CD217)
Cell Type
Th17, Mast cells, Basophils, Epithelial cells, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.

Gene ID
257630 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01/23/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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