Biotin anti-Neurofilament H M (NF-H/NF-M), Hypophosphorylated - anti-Neurofilament H M (NF-H/NF-M), Phospho

Pricing & Availability

Clone: SMI 35 (See other available formats)
Regulatory Status: RUO
Other Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

SMI-35_Biotin_NeurofilamentHM_Antibody_WB_032117_final — Western blot of Biotin anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated antibody (clone SMI 35). Lane 1: Molecular Weight Marker, Lane 2: 20 µg of normal human brain lysate. The blot was incubated with the biotin conjugated antibody at 5 µg/mL for 2 hours at room temperature. Enhanced chemiluminescence was used as the detection system.

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

835606

100 µg

$300

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Neurofilaments (NFs) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest neurofilament (NF-L) runs at 68-70 kD. The medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats of this clone): include ELISA Capture^1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)^6,7.

Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.

Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
RRID: AB_2650681 (BioLegend Cat. No. 835606)

Antigen Details

Structure: The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
Distribution: Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular Distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Interaction: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments, Phospho-Proteins
Antigen References: Petzold A. 2012. Mult Scler Int. 2012:217802. PubMed

Gresle MM, et al. 2011. Mult Scler Int. 2011: 315406. PubMed
Gene ID: 4744 View all products for this Gene ID
UniProt: View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Western Blotting Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Neurofilament H&M (NF-H/NF-M), Phospho Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,WB,IHC-F,ELISA
Purified anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,WB,IHC-F
Biotin anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	WB
Alexa Fluor® 594 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,ICC
Alexa Fluor® 647 anti NF-H/NF-M, Hypophosphorylated	SMI 35	IHC-P
Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,SB

Certificate of Analysis

Go To Top Version: 2 Revision Date: 01/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.