Western blot of Biotin anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate; The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Western blot of Biotin anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate; The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
IHC staining of Biotin anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Biotin anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution
Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.
Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
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