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Biotinylated Recombinant Human CD80-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
B7.1, B7-1, CD80 Antigen, LAB7, BB1, B7, CD28LG1, CD28LG
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Product Citations
publications
a. Biotin-Recom_Human-CD80-Fc-Chimera_1_100724
Biotinylated recombinant human CD80-Fc chimera binds to immobilized recombinant human CTLA-4 (Cat. No. 591902) at 1 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.0 ng/mL.
  • a. Biotin-Recom_Human-CD80-Fc-Chimera_1_100724
    Biotinylated recombinant human CD80-Fc chimera binds to immobilized recombinant human CTLA-4 (Cat. No. 591902) at 1 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.0 ng/mL.
  • b. Biotin-Recom_Human-CD80-Fc-Chimera_2_100724
    Stability Testing for Biotinylated Recombinant Human CD80-Fc Chimera. Biotinylated recombinant human CD80-Fc chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to bind to immobilized recombinant human CTLA-4 (Cat. No. 591902) at 1 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.0 ng/mL.
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584304 25 µg $305
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584306 100 µg $700
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Description

The B7 family of proteins are structurally related, cell membrane-associated molecules that regulate immune responses by delivering costimulatory or coinhibitory signals via their ligands. So far, ten family members have been identified, including B7.1 (CD80), B7.2 (CD86), B7-H1 (PD-L1, CD274), B7-DC (PD-L2, CD273), B7-H2 (ICOSL), B7-H3 (CD276), B7-H, B7-H5, B7-H6, and B7-H7. These proteins are mainly expressed by activated antigen presenting cells (APCs) and when paired with either CD28 or CTLA-4 on a T lymphocyte, they induce an increase or decrease in the downstream signaling activity of the MHC-TCR interaction between APCs and T cells. B7.1 has been shown to bind both CTLA-4 and CD28; however, it has a 20-fold higher affinity to CTLA-4 than to CD28. Despite their structural similarities, the individual contribution of B7.1 and B7.2 to the development of pathogenic T cells in autoimmune diseases and protective T cells in infectious diseases is markedly distinct. Besides regulating T cell immune responses, B7 also plays a role in anti-tumor immunity. This is confirmed by demonstrating that cytotoxic T cells were able to eradicate tumor cells transfected with B7.1 and B7.2. After activation, follicular lymphoma was shown to upregulate B7.1 and other costimulatory and adhesion molecules, therefore increasing APC activity and amplifying primed T cell responses. Homozygous null B7.1/B7.2 mice fail to generate antigen specific IgG1 and IgG2a responses, suggesting that B7 proteins are important in Ig immune responses. Mouse and human B7.1 share 44% amino acid identity.

Product Details
Technical data sheet

Product Details

Source
Human CD80, amino acid sequence (Val35-Asn242) (Accession: # NP_005182.1), was expressed in CHO cells. The carboxy-terminus contains IEGRMD-hIgG1-(Pro100-Lys330)-Fc-Avi tag. Human CD80-Fc-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 468 amino acid recombinant protein has a predicted molecular mass of approximately 56.88 kD. The DTT-reduced and non-reduced proteins migrate at approximately 62 – 80 kD and 140 - 240 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg of cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human CD80-Fc chimera binds to immobilized recombinant human CTLA-4 (Cat. No. 591902) at 1 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.0 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Activated B cells, monocytes, macrophages, dendritic cells

Function
T cell activation as co-stimulatory molecule
Interaction
T cells
Ligand/Receptor
CD28, CTLA-4
Bioactivity
Biotinylated recombinant CD80-Fc chimera binds to CTLA-4
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Pereira JA, et al. 2023. Front Immunol. 14:997376.  
  2. Park S, et al. 2023. Methods Mol Biol. 2654:327-343.  
  3. de Winde CM, et al. 2018. J Cell Sci. :131:jcs214551.  
  4. Park S, et al. 2019. Sci Rep. 9:13286. 
  5. Park S, et al. 2020. Biosens Bioelectron. 165:112389.
  6. Vaccari M, et al. 2019. Front Immunol. 10:1072.  
  7. Pittman M, et al. 2018. J Immunol. 200:586-594.
Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org
Go To Top Version: 1    Revision Date: 10/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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