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Biotinylated Recombinant Human HER2-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
ErbB2, Neu, CD340, TKR1, Herstatin, Proto-Oncogene Neu, human epidermal growth factor receptor 2, Receptor Tyrosine-Protein Kinase ErbB-2.
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Product Citations
publications
HUman_Biotinylated_HER2-Fc_RECOM_1_121521.png
Biotinylated recombinant human HER2-Fc counteracts the inhibition of SK-BR-3 cell proliferation in the presence of 0.12 µg/mL anti-human ErbB2/HER2 (clone 4D5-8, trastuzumab) antibody. The ED50 for this effect is 0.1 – 0.6 µg/mL.
  • HUman_Biotinylated_HER2-Fc_RECOM_1_121521.png
    Biotinylated recombinant human HER2-Fc counteracts the inhibition of SK-BR-3 cell proliferation in the presence of 0.12 µg/mL anti-human ErbB2/HER2 (clone 4D5-8, trastuzumab) antibody. The ED50 for this effect is 0.1 – 0.6 µg/mL.
  • HUman_Biotinylated_HER2-Fc_RECOM_2_121521.png
    Stability Testing for Biotinylated Recombinant Human HER2-Fc Chimera. Biotinylated Recombinant Human HER2-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to counteract the inhibition of SK-BR-3 cell proliferation in the presence of 0.12 µg/mL anti-human ErbB2/HER2 (clone 4D5-8, trastuzumab) antibody. The ED50 for this effect is 0.1 – 0.6 µg/mL.
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796504 25 µg $229
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796506 100 µg $633
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

ErbB2 (HER2) is a member of the ErbB family of receptor tyrosine kinases (RTKs). This family includes EGF receptor, ErbB2, ErbB3, and ErbB4. The protein tyrosine kinases (PTKs) catalyze the transfer of the γ-phosphoryl group of ATP to tyrosine. ErbB2 contains an extracellular domain of 617 amino acids, a transmembrane α-helix, and cytoplasmic domain of 570 amino acids that contains a tyrosine kinase domain and a carboxy-terminal regulatory sequence. The binding of EGF to EGFR and neuregulin to ErbB4 induces dimerization and subsequent activation of the tyrosine kinase domain and finally, autophosphorylation of the ErbB receptors. Ligands for ErbB2 have not been described. ErbB2 can homodimerize constitutively or form heterodimers with other ErbB receptors after their binding to related ligands. In addition, ErbB2 forms homodimers that are not ligand-dependent. ErbB2 mediates different downstream signaling pathways depending on its homodimerization or heterodimerization. The ErbB2 gene is amplified in 20% of the cases of invasive breast cancer and also overexpressed in gastric cancer. Herceptin (trastuzumab), a humanized antibody, has been used for the treatment of breast and gastric cancer.  Genomic profiling of different types of tumors have shown amplification of ErbB2 in salivary, vaginal, bladder, endometrial, cervical, and colorectal cancer. 

Product Details
Technical data sheet

Product Details

Source
Human HER2, amino acid (Gln24-Leu651) (Accession: NM_004448.2), with a C-terminal human IgG1 (Pro100-Lys330), and an Avi-tag, was expressed in CHO cells. Human HER2-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 891 amino acid recombinant protein has a predicted molecular mass of approximately 98.3 kD. The protein migrates at approximately 120 kD and 240 kD by SDS-PAGE in DTT-reduced and non-reduced conditions, respectively. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human HER2 is measured by its ability to block anti-human ErbB2/HER2 (clone 4D5-8, trastuzumab) antibody inhibition of SK-BR-3 cell proliferation. The ED50 for this effect is 0.1 – 0.6 µg/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our <a href="https://www.biolegend.com/ordering#guaranteed" target="_blank">100% satisfaction guarantee</a>. If you have any concerns, contact us at <a href="mailto:tech@biolegend.com" target="_blank" title="Send email to tech@biolegend.com">tech@biolegend.com</a>.

Product Citations
  1. Cheng K, et al. 2023. Int J Mol Sci. 24:. PubMed

Antigen Details

Structure
Dimer
Distribution

Many tissues, overexpressed in breast cancers and gastric cancer

Function
Cell development, proliferation, survival, and motility
Interaction
HER2 (ErbB2) forms dimers with EGFR, ErbB2, ErbB3, and ErbB4
Ligand/Receptor
No ligand is known for ErbB2
Bioactivity
Human HER2 blocks the activity of anti-human ErbB2/HER2 (clone 4D5-8, trastuzumab) antibody inhibition of SK-BR-3 cell proliferation.
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
Molecular Family
CD Molecules, Soluble Receptors
Antigen References
  1. Olayioye MA. 2001. Breast Cancer Res. 3:385.
  2. Cho HS, et al. 2003. Nature. 421: 756
  3. Garret TP, et al. 2003. Mol. Cell. 11: 495.
  4. Bublil EM, Yarden Y. 2007. COCEBI. 19:124.
  5. MA Lemmon. 2009. Exp. Cell. Res. 315:638.
  6. Mitri Z, et al. 2012. Chemother. Res. Pract. 2012:743193.
  7. Sawyer CL. 2019. Cell. 179: 8.
  8. Maddi H, et al. 2021. Cancer (Basel). 13:3540.
  9. Meric-Bernstam F, et al. 2019. Clin. Cancer Res. 25:2033.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about HER2 on UniProt.org
Go To Top Version: 1    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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