Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL $65
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Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical data sheet

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested

Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  2. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  3. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  4. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  5. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  6. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  7. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  8. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  9. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  10. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  11. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  12. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  13. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  14. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  15. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  16. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  17. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  18. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  19. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  20. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  21. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  22. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  23. Chan L, et al. 2022. Cells. 12: . PubMed
  24. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  25. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  26. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  27. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  28. Zander R, et al. 2022. Immunity. 55:475. PubMed
  29. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  30. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  31. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  32. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  33. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  34. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  35. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  36. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  37. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  38. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  39. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  40. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  41. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  42. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  43. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  44. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  45. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  46. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  47. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  48. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  49. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  50. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  51. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  52. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  53. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  54. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  55. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  56. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  57. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  58. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  59. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  60. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  61. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  62. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  63. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  64. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  65. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  66. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  67. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  68. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  69. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  70. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  71. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  72. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  73. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  74. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  75. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  76. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  77. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  78. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  79. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  80. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  81. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  82. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  83. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  84. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  85. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  86. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  87. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  88. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  89. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  90. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  91. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  92. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  93. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  94. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  95. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  96. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  97. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  98. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  99. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  100. Jondle CN, et al. 2021. MBio. 12:. PubMed
  101. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  102. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  103. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  104. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  105. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  106. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  107. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  108. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  109. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  110. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  111. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  112. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  113. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  114. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  115. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  116. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  117. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  118. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  119. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  120. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  121. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  122. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  123. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  124. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  125. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  126. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  127. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  128. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  129. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  130. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  131. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  132. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  133. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  134. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  135. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  136. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  137. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  138. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  139. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  140. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  141. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  142. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  143. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  144. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  145. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  146. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  147. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  148. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  149. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  150. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  151. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  152. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  153. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  154. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  155. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  156. Harty N 2011. J Immunol. 186:3836. PubMed
  157. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  158. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  159. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  160. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  161. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  162. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  163. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  164. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  165. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  166. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  167. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  168. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  169. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  170. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  171. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  172. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  173. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  174. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  175. Ma K, et al. 2022. iScience. 25:104347. PubMed
  176. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  177. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  178. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  179. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  180. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  181. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  182. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  183. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  184. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  185. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  186. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  187. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  188. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  189. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  190. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  191. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  192. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  193. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  194. Sekine T, et al. 2020. Cell. 183:158. PubMed
  195. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  196. Guo C, et al. 2018. Immunity. 49:842. PubMed
  197. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  198. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  199. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  200. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  201. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  202. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  203. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  204. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  205. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  206. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  207. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  208. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  209. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  210. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  211. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  212. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  213. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  214. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  215. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  216. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  217. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  218. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  219. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  220. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  221. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  222. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  223. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  224. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  225. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  226. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  227. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  228. Markey K, et al. 2009. Blood. 113:5644. PubMed
  229. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  230. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  231. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  232. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  233. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  234. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  235. Rist M, et al. 2015. J Virol. 89:703. PubMed
  236. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  237. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  238. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  239. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  240. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  241. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  242. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  243. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  244. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  245. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  246. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  247. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  248. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  249. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  250. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  251. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  252. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  253. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  254. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  255. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  256. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  257. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  258. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  259. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  260. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  261. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  262. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  263. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  264. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed
  265. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  266. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  267. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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