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Brilliant Violet 421™ anti-β-actin Antibody

Pricing & Availability
Clone
2F1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2F1-1_BV421_B-actin_Antibody_1_110519
NIT/3T3 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% triton X-100 for ten minutes followed by blocking with 5% FBS for 30 minutes. The cells were stained with 2.5 µg/mL of anti-β-actin antibody (clone 2F1-1) Brilliant Violet 421™ (blue) in blocking buffer overnight at 4°C. Nuclei were counterstained with DRAQ7 and are shown in red. The image was captured with a 40X objective.
  • 2F1-1_BV421_B-actin_Antibody_1_110519
    NIT/3T3 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% triton X-100 for ten minutes followed by blocking with 5% FBS for 30 minutes. The cells were stained with 2.5 µg/mL of anti-β-actin antibody (clone 2F1-1) Brilliant Violet 421™ (blue) in blocking buffer overnight at 4°C. Nuclei were counterstained with DRAQ7 and are shown in red. The image was captured with a 40X objective.
Compare all formats See Brilliant Violet 421™ spectral data
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643806 100 µg $435
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.
  4. KishidaT,et al. 2007.J. Immunol. 179:8554.
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037.
  9. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  10. Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
  11. Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
RRID
AB_2566300 (BioLegend Cat. No. 643806)

Antigen Details

Structure
Actin, cytoplasmic 1
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 11/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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