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Brilliant Violet 421™ anti-human CD27 Antibody

Pricing & Availability
Clone
M-T271 (See other available formats)
Regulatory Status
RUO
Workshop
V 5T CD27.03
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M-T271_BV421_CD27_Antibody_FC_100713
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) Brilliant Violet 421™ (filled histogram) or mouse IgG1 Brilliant Violet 421™ isotype control (open histogram).
  • M-T271_BV421_CD27_Antibody_FC_100713
    Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) Brilliant Violet 421™ (filled histogram) or mouse IgG1 Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
356417 25 tests $190
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356418 100 tests $381
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T cells from a T-ALL patient.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma S, et al. 2011. J. Virol. 85:165. (IHC)
  2. Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
  3. Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
Product Citations
  1. Xu C, et al. 2022. iScience. 25:105123. PubMed
  2. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  3. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  4. Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed
  5. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  6. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  7. Meehan S, et al. 2019. Commun Biol. 2:229. PubMed
  8. Svensson A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183268. PubMed
  9. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  10. Cao Y, et al. 2021. Cell Res. 31:732. PubMed
  11. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  12. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  13. Wang L, et al. 2021. Vaccines (Basel). :9. PubMed
  14. Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed
  15. Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
  16. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  17. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  18. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  19. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  20. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
  21. Heiden M, et al. 2016. Sci Rep. 6: 26892. PubMed
  22. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
RRID
AB_2562598 (BioLegend Cat. No. 356417)
AB_2562598 (BioLegend Cat. No. 356418)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Interaction
CD27 binds to CD70
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 10/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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