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Brilliant Violet 421™ anti-human CD288 (TLR8) Antibody

Pricing & Availability
Clone
S16018A (See other available formats)
Regulatory Status
RUO
Other Names
CD288, Toll-like receptor 8
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S16018A_BV421_CD288_Antibody_082020.png
Human peripheral blood monocytes were stained with CD14 APC, treated with BioLegend’s Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with CD288 (TLR8) (clone S16018A) Brilliant Violet 421™ (left) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (right). Data shown is gated on the monocyte population.
  • S16018A_BV421_CD288_Antibody_082020.png
    Human peripheral blood monocytes were stained with CD14 APC, treated with BioLegend’s Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with CD288 (TLR8) (clone S16018A) Brilliant Violet 421™ (left) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (right). Data shown is gated on the monocyte population.
Compare all formats See Brilliant Violet 421™ spectral data
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395509 25 tests $215
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395510 100 tests $435
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Description

Toll-like receptor (TLR) 8, also known as CD288, is a 83 kDa, type 1 transmembrane protein, member of the TLR receptor family. TLR8 is expressed intracellulary in monocytes, macrophages and myeloid dendritic cells, and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR8 triggers anti-viral reponses promoting the secretion of proinflammatory cytokines such as IL-12 and TNF.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR8-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
RRID
AB_2876712 (BioLegend Cat. No. 395509)
AB_2876712 (BioLegend Cat. No. 395510)

Antigen Details

Structure
83 kD, Type 1 transmembrane protein
Distribution

Endosomal compartments of monocytes, macrophages, myeloid dendritic cells, and neutrophils

Function
Binds single-stranded RNA
Interaction
MYD88, TRAF6, IRAK4
Ligand/Receptor
Single-stranded GU-rich RNA
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References
  1. Lood C, et al. 2017. J Exp Med. 214:2103.
  2. Prinz N, et al. 2011. Blood. 118:2322.
  3. Gringhuis SI, et al. 2010. Nat Immunol. 11419.
  4. Cros J, et al. 2010. Immunity. 33:375.
  5. Peng G, et al. 2005. Science. 309:1380.
Gene ID
51311 View all products for this Gene ID
UniProt
View information about CD288 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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