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Brilliant Violet 421™ anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A7R34_BV421_1_040311
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD127 (clone A7R34) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A7R34_BV421_1_040311
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD127 (clone A7R34) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A7R34_BV421_2_040311
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
135023 125 µL $215
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135027 50 µg $242
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135024 500 µL $413
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
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  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  5. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  6. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  7. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  8. Campisi L, et al. 2022. Nature. 606:945. PubMed
  9. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
  10. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  11. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  12. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  13. Xu W 2023. Cell Reports. 42(1):111987. PubMed
  14. Xu R 2023. Cell Reports. 42(2):112054. PubMed
  15. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  16. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  17. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  18. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
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  22. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
  23. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  24. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  25. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  26. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  27. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  28. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  29. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
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  31. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  32. Ma EH, et al. 2020. Immunity. 51(5):856-870. PubMed
  33. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
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  35. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  36. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  37. Cha SE, et al. 2021. Oncoimmunology. 10:1899469. PubMed
  38. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  39. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  40. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  41. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  42. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  43. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  44. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  45. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  46. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  47. Sheldon RD, et al. 2021. Nat Protoc. 16:4494. PubMed
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  50. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  51. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  52. Siegemund S, et al. 2015. PLoS One. 10:124661. PubMed
  53. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  54. Limbach K, et al. 2017. Malar J. 10.1186/s12936-017-1911-z. PubMed
  55. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  56. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  57. Abdelsamed HA, et al. 2020. Nat Immunol. 1.276388889. PubMed
  58. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  59. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  60. Huang X, et al. 2021. Immunity. . PubMed
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RRID
AB_10897948 (BioLegend Cat. No. 135023)
AB_10897948 (BioLegend Cat. No. 135027)
AB_10897948 (BioLegend Cat. No. 135024)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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