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Brilliant Violet 421™ anti-mouse CD186 (CXCR6) Antibody

Pricing & Availability
Clone
SA051D1 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR6, Chemokine (C-X-C motif) receptor 6, BONZO, STRL33, chemokine receptor 6, CD 186, CXC-R6, CXCR-6, C-X-C chemokine receptor type 6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA051D1_BV421_CD186_Antibody_1_041416
C57BL/6 mouse splenocytes were stained with CD3 PE and CD186 (clone SA051D1) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • SA051D1_BV421_CD186_Antibody_1_041416
    C57BL/6 mouse splenocytes were stained with CD3 PE and CD186 (clone SA051D1) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • SA051D1_BV421_CD186_Antibody_2_041416
Compare all formats See Brilliant Violet 421™ spectral data
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151109 50 µg $292
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Description

CD186, or CXCR6, is a 39 kD G-protein coupled chemokine receptor with seven transmembrane-spanning regions. Its ligand is CXCL16. It is expressed on different T cell subsets and is upregulated in activated T cells. Expression of CXCR6 is correlated with increased inflammatory responses and seems to contribute to liver fibrosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR6-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  4. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  5. Muthuswamy R, et al. 2021. J Immunother Cancer. 9:. PubMed
  6. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  7. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  8. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  9. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  10. Wang B, et al. 2021. J Immunother Cancer. 9:. PubMed
  11. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
RRID
AB_2616760 (BioLegend Cat. No. 151109)

Antigen Details

Structure
G-protein coupled receptor 1 family.
Distribution

T cells and natural killer T (NKT) cells.

Function
Chemotaxis and the development of T cells.
Ligand/Receptor
CXCL16.
Cell Type
NKT cells, T cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kim CH, et al. 2001. J. Clin. Invest. 107:595.
2. Heesch K, et al. 2014. PLoS One. 9:5.
3. Wehr A, et al. 2013. J. Immunol. 190:5226.

Gene ID
80901 View all products for this Gene ID
UniProt
View information about CD186 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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