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Brilliant Violet 421™ anti-mouse CD24 Antibody

Pricing & Availability
Clone
M1/69 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1slash69_BV421_062311
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Brilliant Violet 421™ (filled histogram) or with rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
  • M1slash69_BV421_062311
    C57BL/6 splenocytes were stained with CD24 (clone M1/69) Brilliant Violet 421™ (filled histogram) or with rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
101825 125 µL $171
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101826 50 µg $237
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Description

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  2. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  3. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  4. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  5. Cayre S, et al. 2020. Development. 147:00:00. PubMed
  6. Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed
  7. Cui Z, et al. 2022. Bone Res. 10:58. PubMed
  8. Morales RA, et al. 2022. Mucosal Immunol. 15:940. PubMed
  9. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  10. Romagnoli M, et al. 2020. Development. 147:. PubMed
  11. Romagnoli M, et al. 2019. Stem Cell Reports. 1.077083333. PubMed
  12. Bresson L, et al. 2018. Development. 145. PubMed
  13. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  14. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  15. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  16. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  17. Cherukuri A, et al. 2014. J Am Soc Nephrol. 7:1575. PubMed
  18. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  19. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  20. Cayre S, et al. 2020. Development. . PubMed
RRID
AB_10901159 (BioLegend Cat. No. 101825)
AB_10901159 (BioLegend Cat. No. 101826)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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