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Brilliant Violet 421™ anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a-RM4-5_BV421_1_070611
C57BL/6 mouse splenocytes were stained with CD3 APC and CD4 (clone RM4-5) Brilliant Violet 421™ (top), or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • a-RM4-5_BV421_1_070611
    C57BL/6 mouse splenocytes were stained with CD3 APC and CD4 (clone RM4-5) Brilliant Violet 421™ (top), or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • b-RM4-5_BV421_2_070611
  • c-RM4-5_BV421_CD4_Antibody_IF_100119
    C57BL/6 mouse thymocytes were fixed with 1% paraformaldehyde (PFA), and then stained with 5 µg/ml of CD4 (clone RM4-5) Brilliant Violet 421™ (blue) for 30 minutes at room temperature. Nuclei were counterstained with Propidium Iodide and are shown in red. The image was captured by 40X objective.
  • d-RM4-5_BV421_CD4_Antibody_IHC-F_100119
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD4 (clone RM4-5) Brilliant Violet 421™ (blue), anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (green), and anti-mouse CD8b (clone YTS156.7.7) Alexa Fluor® 647 (red) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data
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100543 125 µL $171
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100563 50 µg $274
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100544 500 µL $380
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry staining using µl sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry on frozen tissue sections using the µg size, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  3. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  4. Kim E, et al. 2022. Immunity. 55:145. PubMed
  5. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  6. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  7. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  8. Lechuga-Vieco AV, et al. 2020. Sci Adv. 6:eaba5345. PubMed
  9. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  10. Prasad M, et al. 2020. Cell Mol Immunol. . PubMed
  11. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  12. Wang D, et al. 2018. Immunity. 48:659. PubMed
  13. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  14. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  15. Park S, et al. 2021. Front Immunol. 11:620166. PubMed
  16. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  17. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  18. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  19. Stein RR, et al. 2018. Elife. 7:e30916. PubMed
  20. Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed
  21. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  22. Köchl R, et al. 2020. Elife. 9:00. PubMed
  23. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  24. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  25. Parayath NN, et al. 2020. Nat Commun. 4.680555556. PubMed
  26. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  27. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  28. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  29. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  30. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  31. Chen EW, et al. 2018. Sci Rep. 8:10046. PubMed
  32. Kim D, et al. 2020. Immunity. 53(3):581-596.e5. PubMed
  33. Lopes N, et al. 2022. Elife. 11:. PubMed
  34. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  35. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  36. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  37. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  38. Stone S et al. 2018. Glia. 66(7):1331-1345 . PubMed
  39. Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
  40. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  41. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
RRID
AB_11219790 (BioLegend Cat. No. 100543)
AB_11219790 (BioLegend Cat. No. 100563)
AB_11219790 (BioLegend Cat. No. 100544)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 6    Revision Date: 10/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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