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Brilliant Violet 510™ anti-mouse CD83 Antibody

Pricing & Availability
Clone
Michel-19 (See other available formats)
Regulatory Status
RUO
Other Names
HB15
Isotype
Rat IgG1, κ
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Product Citations
publications
Michel-19_BV510_CD83_Antibody_1_050924
Mouse CD83 transfected cells were stained with anti-mouse CD83 (clone Michel-19) Brilliant Violet 510™ (filled histogram), or rat IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
  • Michel-19_BV510_CD83_Antibody_1_050924
    Mouse CD83 transfected cells were stained with anti-mouse CD83 (clone Michel-19) Brilliant Violet 510™ (filled histogram), or rat IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
Compare all formats See Brilliant Violet 510™ spectral data
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121527 50 µg $270
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Description

CD83 is a 45 kD type I transmembrane protein. It belongs to immunoglobulin superfamily and is expressed on mature dendritic cells and activated lymphocytes. CD83 is involved in the regulation of T cell development and immune response. Soluble form CD83 has been reported to inhibit dendritic cell maturation and dendritic cell-mediated T cell proliferation. Murine CD83 ligand has been found on B cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD83 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
  2. Fujimoto Y, et al. 2002. Cell 108:755.
  3. Mott KR, et al. 2009. Virol J. 6:56. (FC) PubMed
  4. Roland Cl, et al. 2009. Mol Cancer Res. 8:1761. (IHC) PubMed
  5. Masuda Y, et al. 2010. Cancer Immunol Immunother. [Epub ahead of print] (FC) PubMed
  6. Tze LE, et al. 2011. J Exp Med. PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_3662362 (BioLegend Cat. No. 121527)

Antigen Details

Structure
Type I transmembrane protein, Ig superfamily, 45kD
Distribution

Mature dendritic cells, activated lymphocytes

Function
Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
Ligand/Receptor
CD83 ligand
Cell Type
Dendritic cells, Lymphocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132.
2. Kotxor N, et al. 2004. Immunobiology 209:129.
3. Leon F, et al. 2004. J. Immunol. 173:2995.
4. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
5. Fujimoto Y, et al. 2002. Cell 108:755.

Gene ID
12522 View all products for this Gene ID
UniProt
View information about CD83 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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