Brilliant Violet 510™ anti-mouse H-2K<sup>b</sup> Antibody, H-2Kb, AF6-88.5

Pricing & Availability

Clone: AF6-88.5 (See other available formats)
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG2a, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with H-2Kb (clone AF6-88.5) Brilliant Violet 510™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 510™ isotype control (open histogram).

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116523

50 µg

$245

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Description

The AF6-88.5 antibody reacts with H-2K^b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K^b haplotype. H-2K^b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunohistochemical staining of acetone-fixed frozen sections³. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
RRID: AB_2800584 (BioLegend Cat. No. 116523)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All H-2Kb Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse H-2K^b	AF6-88.5	FC
FITC anti-mouse H-2K^b	AF6-88.5	FC
PE anti-mouse H-2K^b	AF6-88.5	FC
Purified anti-mouse H-2K^b	AF6-88.5	FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse H-2K^b	AF6-88.5	FC
Alexa Fluor® 647 anti-mouse H-2K^b	AF6-88.5	FC
Pacific Blue™ anti-mouse H-2K^b	AF6-88.5	FC
PerCP/Cyanine5.5 anti-mouse H-2K^b	AF6-88.5	FC
APC anti-mouse H-2K^b	AF6-88.5	FC
PE/Cyanine7 anti-mouse H-2K^b	AF6-88.5	FC
Alexa Fluor® 700 anti-mouse H-2K^b	AF6-88.5	FC
Brilliant Violet 510™ anti-mouse H-2K^b	AF6-88.5	FC
Brilliant Violet 421™ anti-mouse H-2K^b	AF6-88.5	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02/21/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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