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Brilliant Violet 570™ anti-human CD33 Antibody

Pricing & Availability
Clone
WM53 (See other available formats)
Regulatory Status
RUO
Workshop
IV M-505
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM53_BV570_100311
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
  • WM53_BV570_100311
    Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Compare all formats See Brilliant Violet 570™ spectral data
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303417 25 tests $226
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myeloid leukaemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
  3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
  4. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Vanuytsel K, et al. 2022. Cytometry A. 101:903. PubMed
  2. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  3. Ogishi M, et al. 2022. J Exp Med. 219:. PubMed
  4. Thompson EA, et al. 2021. Cell Rep. 108863:34. PubMed
RRID
AB_10900071 (BioLegend Cat. No. 303417)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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