- Clone
- L17F12 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- II T7
- Other Names
- Leu-1, Ly-1, T1, Tp67, Leu1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L17F12_BV605_CD5_Antibody_FC_072015 L17F12_BV605_CD5_Antibody_FC_072015](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L17F12_BV605_CD5_Antibody_FC_072015.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) Brilliant Violet 605™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
364019 | 25 tests | $198 | ||||
364020 | 100 tests | $418 |
CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human T-acute lymphoblastic leukemia (ALL) cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats): immunoprecipitation1,3,4 and immmunohistochemical staining of frozen and formalin-fixed paraffin-embedded sections2,3,5.
-
Application References
(PubMed link indicates BioLegend citation) -
- McAlister MS, et al. 1998. Protein Eng. 11:847. (IP)
- Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC)
- Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP)
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP)
- Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)
- Product Citations
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- RRID
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AB_2565940 (BioLegend Cat. No. 364019)
AB_2565941 (BioLegend Cat. No. 364020)
Antigen Details
- Structure
- Scavenger receptor superfamily, 67 kD
- Distribution
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Thymocytes, T cells, and subsets of B and dendritic cells.
- Function
- Modulates TCR and BCR signaling, involved in thymocyte maturation and T-B cell interaction
- Ligand/Receptor
- CD72
- Cell Type
- B cells, Dendritic cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kipps T. 1989. Adv. Immunol. 47:117.
2. Resnick D, et al. 1994. Trends Biochem. Sci. 1:5.
3. Wood GS and Freudenthal PS. 1992. Am. J. Pathol. 141:789. - Gene ID
- 921 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE/Cyanine7 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Purified anti-human CD5
Human peripheral blood lymphocytes were stained with purifie... Human frozen tonsil section was fixed with 4% paraformaldehy... -
Biotin anti-human CD5
Human peripheral blood lymphocytes were stained with biotiny... -
PerCP/Cyanine5.5 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
APC/Cyanine7 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
PE/Dazzle™ 594 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
PE anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Brilliant Violet 510™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
APC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Brilliant Violet 605™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
FITC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Pacific Blue™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Alexa Fluor® 700 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
APC/Fire™ 750 anti-human CD5
Human peripheral blood lymphocytes were stained with APC/Fir... -
Brilliant Violet 421™ anti-human CD5
Human peripheral blood lymphocytes were stained with Brillia... -
PE anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine5 anti-human CD5
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
FITC anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
Pacific Blue™ anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD5
Human peripheral blood cells were surface stained with anti-... -
PE/Fire™ 810 anti-human CD5
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC/Fire™ 750 anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD5
Typical results from human peripheral blood lymphocytes stai... -
GMP Pacific Blue™ anti-human CD5
Typical results from human peripheral blood lymphocytes stai...
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