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Brilliant Violet 605™ anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/23 (See other available formats)
Regulatory Status
RUO
Other Names
5'-nucleotidase, Nt5e, 5'-NT, Ecto-5'-nucleotidase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TY23_BV605_CD73_Antibody_061423
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD73 (clone TY/23) Brilliant Violet 605™ (left) or rat IgG2a, κ Brilliant Violet 605™ isotype control (right).
  • TY23_BV605_CD73_Antibody_061423
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC and anti-mouse CD73 (clone TY/23) Brilliant Violet 605™ (left) or rat IgG2a, κ Brilliant Violet 605™ isotype control (right).
Compare all formats See Brilliant Violet 605™ spectral data
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117205 50 µg $292
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Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse splenocytes and CHO cells transfected with the mouse CD73 gene, Nt5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The TY/23 antibody has been reported to inhibit the enzymatic activity of CD73.

Application References

(PubMed link indicates BioLegend citation)

1. Zhang B. 2010. Cancer Res. 70:6407-11.  
2. Stagg J, et al. 2010. Proc Natl Acad Sci U S A. 107:1547-52.
3. Forte G, et al. 2012. J Immunol. 189:2226-33.

RRID
AB_3083107 (BioLegend Cat. No. 117205)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Yamashita Y, et al. 1998. Eur J Immunol. 28:2981-90.
  2. Resta R, et al. 1998. Immunol Rev. 161:95-109.
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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