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Brilliant Violet 605™ anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/11.8 (See other available formats)
Regulatory Status
RUO
Other Names
Ecto-5'-nucleotidase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TY11.8_BV605_CD73_Antibody_FC_1_083112
C57BL/6 mouse splenocytes were stained with CD3 APC and CD73 (clone TY/11.8) Brilliant Violet 605™.
  • TY11.8_BV605_CD73_Antibody_FC_1_083112
    C57BL/6 mouse splenocytes were stained with CD3 APC and CD73 (clone TY/11.8) Brilliant Violet 605™.
Compare all formats See Brilliant Violet 605™ spectral data
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127215 125 µL $215
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Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
  2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations
  1. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  2. Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
  3. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  4. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  5. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  6. Perrot I et al. 2019. Cell Rep. 27(8):2411-2425 . PubMed
  7. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  8. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  9. Quiroz HJ, et al. 2022. Front Cardiovasc Med. 8:826687. PubMed
  10. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  11. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  12. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  13. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  14. Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed
RRID
AB_2561528 (BioLegend Cat. No. 127215)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Regulation
Expression is regulated by the development and maturation of leukocytes
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org
Go To Top Version: 2    Revision Date: 01/03/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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