Brilliant Violet 605™ anti-mouse MERTK (Mer) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse MERTK extracellular domain

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Product Citations

1. DiGiovanni GT, et al. 2023. JCI Insight. :8. PubMed

RRID

AB_2876506 (BioLegend Cat. No. 151517)

Antigen Details

Structure

Member of the TAM (TYRO3/AXL/MerTK) family. Encodes a transmembrane protein, two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.

Distribution

Macrophages, monocytes, and dendritic cells.

Function

Regulates TLR signaling, efferocytosis, phagocytosis, cell survival, and macrophage migration. Inhibits production of inflammatory cytokines.

Interaction

Grb2, LimD4, and Vav1.

Ligand/Receptor

LGALS3, TUB, TULP1, and GAS6.

Cell Type

Dendritic cells, Macrophages, Monocytes

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

Toll Like Receptors

Antigen References

1. Zagórska A, et al. 2014. Nat. Immunol. 15:920.
2. Toda S, et al. 2014. Blood 123:3963.
3. Chung WS, et al. 2013. Nature 504:394.
4. Carrera Silva EA, et al. 2013. Immunity 39:160.
5. Yi Z, et al. 2009. Blood 114:3191.

Gene ID

17289 View all products for this Gene ID

UniProt

View information about MERTK on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis