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Brilliant Violet 650™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 650™
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Product Citations
publications
SAV_BV650_043012
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.
  • SAV_BV650_043012
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.
See Brilliant Violet 650™ spectral data
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405231 100 µL $231
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405232 100 µg $462
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 650™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 650™ by 405 nm laser light induces a fluorescence maximum emission of 645 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405231: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Cat. No. 405232: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Cat. No. 405231: 0.1 mg/ml
Cat. No. 405232: 0.5 mg/ml
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 650™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 650™ conjugation. For immunofluorescent staining, we recommend using ≤0.125 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 650™ is 340 kD and Streptavidin alone is 52 kD.

Product Citations
  1. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  2. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  3. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  4. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  5. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  6. Caradonna TM, et al. 2022. Cell Rep. 41:111628. PubMed
  7. Gong M, et al. 2023. iScience. 26:106774. PubMed
  8. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  9. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  10. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  11. Williams B, et al. 2015. Dis Model Mech. 8: 805-815. PubMed
  12. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  13. Guo C, et al. 2021. Cell Rep Med. 2:100448. PubMed
  14. Kitajima Y, et al. 2022. Cells. 11:. PubMed
  15. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  16. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  17. Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed
  18. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  19. Yamamoto T, et al. 2015. Sci Transl Med. 7: 298ra120. PubMed
  20. Turner JS, et al. 2021. Nature. 595:421. PubMed
  21. Martin S, et al. 2021. iScience. 24:103185. PubMed
  22. Wells DK, et al. 2020. Cell. 183:818. PubMed
  23. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  24. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  25. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
Go To Top Version: 5    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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