- Clone
- 9E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E9_BV711_CD16dot2_Antibody_050917 9E9_BV711_CD16dot2_Antibody_050917](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E9_BV711_CD16dot2_Antibody_050917.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with CD16.2 (clone 9E9) Brilliant Violet 711™ (filled histogram) or Armenian hamster IgG Brilliant Violet 711™ isotype control (open histogram). Data shown was gated on the myeloid population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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149529 | 50 µg | $286 |
FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- FCγR4 —EC domain fusion with IgG1 Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
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Application References
(PubMed link indicates BioLegend citation) -
- Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
- Nimmerjahn F, et al. 2005. Immunity 23:41.
- RRID
-
AB_2687226 (BioLegend Cat. No. 149529)
Antigen Details
- Structure
- 22.4 kD
- Distribution
-
Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
- Function
- Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
- Ligand/Receptor
- IgG2a and IgG2b
- Cell Type
- B cells, Leukocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
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- Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
- Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
- Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
- Gene ID
- 246256 View all products for this Gene ID
- UniProt
- View information about CD16.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD16.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD16.2 (FcγRIV) | 9E9 | FC,IHC-F |
PE anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Biotin anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
FITC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with purified CD16.2 ... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse frozen intestine section was fixed with 4% par... -
PE anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
APC anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Biotin anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with biotinylat... -
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
FITC anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with Alexa Fluor... -
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 myeloid cells were stained with anti-mouse CD16.2 (c...
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