Brilliant Violet 750™ anti-human CD273 (B7-DC, PD-L2) Antibody, CD273, MIH18

Pricing & Availability

Clone: MIH18 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B7-DC, PD-L2, PDL2, B7DC
Isotype: Mouse IgG1, κ
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Product Citations: publications

MIH18_BV750_CD273_Antibody_1_092723 — Human monocyte-derived dendritic cells were stained with anti-human CD273 (clone MIH18) Brilliant Violet 750™ (filled histogram) or mouse IgG1, κ Brilliant Violet 750™ isotype control (open histogram).

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345527

25 tests

$209

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345528

100 tests

$424

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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. Clone MIH18 is reported to block PDL2.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human B7-DC transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking^4,5, and immunohistochemistry in frozen sections² and paraffin-embedded formalin-fixed sections⁶. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).
RRID: AB_3083333 (BioLegend Cat. No. 345527)
AB_3083333 (BioLegend Cat. No. 345528)

Antigen Details

Structure: B7 Immunoglobulin superfamily, 25 kD
Distribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages
Function: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor: PD-1
Cell Type: Dendritic cells, Macrophages
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.
Gene ID: 80380 View all products for this Gene ID
UniProt: View information about CD273 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Cancer Immunoediting

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Other Formats

View All CD273 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD273 (B7-DC, PD-L2)	MIH18	FC,IHC-F,IHC-P,Block
PE anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
APC anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Biotin anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
PE/Cyanine7 anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Alexa Fluor® 647 anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
APC/Cyanine7 anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
PE/Dazzle™ 594 anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Brilliant Violet 421™ anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Ultra-LEAF™ Purified anti-human CD273 (B7-DC, PD-L2)	MIH18	FC,IHC-F,IHC-P,Block
Brilliant Violet 650™ anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Brilliant Violet 750™ anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Brilliant Violet 785™ anti-human CD273 (B7-DC, PD-L2)	MIH18	FC
Brilliant Violet 711™ anti-human CD273 (B7-DC, PD-L2)	MIH18	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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