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Cell-Vive™ GMP Recombinant Human GM-CSF (carrier-free)

Pricing & Availability
Other Names
Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
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publications
Human_GM-CSF_CF_GMP-RECOM_1_090220
GMP recombinant human GM-CSF induces dose-dependent proliferation of TF-1 erythroleukemic cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The ED50 range for this effect is 0.01 – 0.04 ng/mL.
  • Human_GM-CSF_CF_GMP-RECOM_1_090220
    GMP recombinant human GM-CSF induces dose-dependent proliferation of TF-1 erythroleukemic cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The ED50 range for this effect is 0.01 – 0.04 ng/mL.
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572914 25 µg $382
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572916 100 µg $1231
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always co-expressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain non-hemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Low endotoxin level (≤ 0.1 EU/μg)
  • Purity (≥ 95% or higher)
  • Bioburden testing
  • Mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical data sheet

Product Details

Source
Human GM-CSF, amino acids Ala18- Glu144 (Accession# NM_000758) was expressed in E. coli.
Molecular Mass
The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.5 kD. The DTT-reduced protein migrates at approximately 14 kD and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.
N-terminal
Sequence Analysis
Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr
Purity
≥ 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.1 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content
≤ 0.500 ng/μg by ELISA
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.01 – 0.04 ng/mL as determined by the dose-dependent stimulation of TF-1 cell proliferation. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The specific activity of Cell-Vive™ GMP Recombinant Human GM-CSF (carrier-free) is ≥ 1.8 x 107 IU/mg when compared against the WHO International Standard for human GM-CSF (NIBSC code: 88/646).
Application

Bioassay
Cell Culture
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Cytokine
Distribution

T cells, macrophages, fibroblasts, endothelial cells, mast cells.

Function
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Interaction
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
Ligand/Receptor
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common
Bioactivity
Measured by its ability to induce proliferation of TF-1 erythroleukemic cells.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Hercus TR, et al. 2009. Blood. 114:1289-98.
  2. Hayashida K, et al. 1990. Proc Natl Acad Sci USA. 87:9655-9.
  3. Walker F & Burgess AW. 1985. EMBO J. 4:933-9.
  4. Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org
Go To Top Version: 2    Revision Date: 08/29/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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