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Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody

Pricing & Availability
Clone
CD28.2 (See other available formats)
Other Names
T44, Tp44
Isotype
Mouse IgG1, κ
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Product Citations
publications
CD28dot2_GMP_ULEAF_BIOTIN_CD28_Antibody_1_052523
Human peripheral lymphocytes were stained with GMP Ultra-LEAF™ Biotin anti-human CD28 SF antibody (clone CD28.2) (filled histogram) or isotype control (clone MOPC-21) (open histogram), followed by Streptavidin-PE stain.
  • CD28dot2_GMP_ULEAF_BIOTIN_CD28_Antibody_1_052523
    Human peripheral lymphocytes were stained with GMP Ultra-LEAF™ Biotin anti-human CD28 SF antibody (clone CD28.2) (filled histogram) or isotype control (clone MOPC-21) (open histogram), followed by Streptavidin-PE stain.
  • CD28dot2_GMP_ULEAF_BIOTIN_CD28_Antibody_2_052523
    PBMC-derived T cells were activated in the presence of GMP Ultra-LEAF™ Biotin anti-human CD28 SF antibody (Cat. No. 302978) alone, GMP Ultra-LEAF™ Biotin anti-human CD3 SF antibody (Cat. No. 317358) alone, or the combination of both antibodies (Cat. No. 302978 and 317358) for a period of 4 days. Activation was done via plate coating, with 1 µg/mL of each antibody. Cells were cultured for a total of 11 days and viable cell number was determined.
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302977 100 µg $578
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302978 1 mg $2970
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major costimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody was GMP manufactured under serum-free conditions including serumfree hybridoma cell culture, without additional animal or humanderived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths.

GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody is for research use only. Please inquire any additional application right by contacting BioLegend.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Ultra-Low endotoxin level (< 0.01 EU/μg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Activation - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research use only.

Additional Product Notes

This antibody is intended for flow cytometry and activation/expansion of T cells for research use only. Please inquire any additional application right by contacting BioLegend.

 

For plate coating protocols, CD28.2 antibody (Cat. No. 302977/302978) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of Biotin anti-human CD3 antibody (Cat. No. 317358) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of antihuman CD3 (Cat. No. 317358) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 302977/302978).

Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBVpositive B cells

Function
T cell costimulation
Ligand/Receptor
CD80/CD86
Cell Type
B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linskey PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD28 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD28 CD28.2 FC
Biotin anti-human CD28 CD28.2 FC
FITC anti-human CD28 CD28.2 FC
PE anti-human CD28 CD28.2 FC
PE/Cyanine5 anti-human CD28 CD28.2 FC
Purified anti-human CD28 CD28.2 FC,IHC-F,Costim,FA
Alexa Fluor® 488 anti-human CD28 CD28.2 FC
Alexa Fluor® 700 anti-human CD28 CD28.2 FC
PerCP/Cyanine5.5 anti-human CD28 CD28.2 FC
Pacific Blue™ anti-human CD28 CD28.2 FC
PE/Cyanine7 anti-human CD28 CD28.2 FC
Ultra-LEAF™ Purified anti-human CD28 CD28.2 FC,IHC-F,Costim,FA
Brilliant Violet 421™ anti-human CD28 CD28.2 FC
Brilliant Violet 510™ anti-human CD28 CD28.2 FC
Purified anti-human CD28 (Maxpar® Ready) CD28.2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD28 CD28.2 FC
Brilliant Violet 785™ anti-human CD28 CD28.2 FC
Brilliant Violet 650™ anti-human CD28 CD28.2 FC
Brilliant Violet 711™ anti-human CD28 CD28.2 FC
APC/Fire™ 750 anti-human CD28 CD28.2 FC
Alexa Fluor® 647 anti-human CD28 CD28.2 FC
TotalSeq™-A0386 anti-human CD28 CD28.2 PG
TotalSeq™-B0386 anti-human CD28 CD28.2 PG
TotalSeq™-C0386 anti-human CD28 CD28.2 PG
Brilliant Violet 605™ anti-human CD28 CD28.2 FC
APC/Cyanine7 anti-human CD28 CD28.2 FC
PE anti-human CD28 CD28.2 FC
APC anti-human CD28 CD28.2 FC
Brilliant Violet 750™ anti-human CD28 CD28.2 FC
PE/Fire™ 810 anti-human CD28 CD28.2 FC
GMP PE anti-human CD28 CD28.2 FC
TotalSeq™-D0386 anti-human CD28 CD28.2 PG
Spark Violet™ 423 anti-human CD28 CD28.2 FC
GMP Ultra-LEAF™ Purified anti-human CD28 SF CD28.2 FC,Costim
GMP APC anti-human CD28 CD28.2 FC
PE/Cyanine7 anti-human CD28 CD28.2 FC
Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD28 SF CD28.2 FC,Activ
PerCP/Cyanine5.5 anti-human CD28 CD28.2 FC
Spark Blue™ 515 anti-human CD28 CD28.2 FC
Spark Red™ 718 anti-human CD28 CD28.2 FC
GMP PerCP/Cyanine5.5 anti-human CD28 CD28.2 FC
Pacific Blue™ anti-human CD28 CD28.2 FC
Go To Top Version: 3    Revision Date: 12/05/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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