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Cell-Vive™ GMP Ultra-LEAF™ Purified anti-HU CD28 RecAb SF

Pricing & Availability
Clone
S20013F.Rec (See other available formats)
Other Names
T44, Tp44
Isotype
Mouse IgG1, κ
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Product Citations
publications
S20013F-Rec_CV_GMP_ULEAF_CD28_RecAb_SF_image1_102324
Human peripheral blood lymphocytes were stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD28 RecAb SF at 0.25 µg per test (Clone S20013F.Rec, cat#: 377614, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400101, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
  • S20013F-Rec_CV_GMP_ULEAF_CD28_RecAb_SF_image1_102324
    Human peripheral blood lymphocytes were stained with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD28 RecAb SF at 0.25 µg per test (Clone S20013F.Rec, cat#: 377614, filled histogram) or an isotype control (Clone MOPC-21, cat#: 400101, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
  • S20013F-Rec_CV_GMP_ULEAF_CD28_RecAb_SF_image2_102324
    PBMC-derived T cells were activated in the presence of Cell-Vive™ GMP Ultra-LEAF™ anti-human CD28 Recombinant SF antibody (S20013F.Rec, cat#: 377614, black bar) alone or combined with Cell-Vive™ GMP Ultra-LEAF™ anti-human CD3 Recombinant SF antibody (clone OKT3.Rec, cat#: 317368, purple bar). These conditions were compared to both: OKT3.Rec combined with Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD28 SF antibody (clone S20013F, cat#: 377605, pink bar) and S20013F.Rec combined with Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 SF Antibody (clone: OKT3, cat#: 317353, teal bar). T cells were activated by plating 1x106 cells on a 12 well plate coated with 1 µg/mL of each antibody in PBS. Cells were cultured for a total of 10 days and fold expansion compared to D0 was calculated at D10. Statistical significance was observed for each antibody pair compared to Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD28 RecAb SF antibody alone. Unpaired t-tests confirmed no significant difference in fold expansion between antibody pairs.
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377613 100 µg $328
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377614 1 mg $1683
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Ultra-Low endotoxin level (<0.01 EU/µg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Activ - Verified in house

Costim - Reported in the literature
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

S20013F.Rec antibody can be used at 1 µg/mL of antibody in PBS in combination with 1 µg/mL of anti-human CD3 antibody for T cell activation.

Application Notes

Clone S20013F.Rec antibody can completely block the binding of clone CD28.2 on target. Clone S20013F.Rec can partially block Clone S20013B on target. Clone S20013F.Rec can increase anti-CD3 induced human peripheral T-cell proliferation.

 

This GMP Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.1 µm filtered) is recommended for highly sensitive assays.

Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

Function
T cell co-stimulation
Ligand/Receptor
CD80/CD86
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212
Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 1    Revision Date: 11/05/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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