- Clone
- 8RB13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
663907 | 100 µL | $340 |
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant E. coli RNA polymerase β subunit.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
-
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3. -
Application References
(PubMed link indicates BioLegend citation) -
- Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
- Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
- Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
- RRID
-
AB_2629625 (BioLegend Cat. No. 663907)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
-
Bacteria.
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-E. coli RNA Polymerase ImmunoAffinity Resin | 8RB13 | IP |
Anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Purified anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Direct-Blot™ HRP anti-E. coli RNA Polymerase β | 8RB13 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-E. coli RNA Polymerase ImmunoAffinity Resin
-
Anti-E. coli RNA Polymerase β
Total cell lysates (15 µg total protein) from E. Coli Total cell lysates (15 µg protein) from E. Coli str... -
Purified anti-E. coli RNA Polymerase β
Total lysates (15 µg protein) from E. coli BL21 cells were r... -
Direct-Blot™ HRP anti-E. coli RNA Polymerase β
Total cell lysates (15 µg protein) from HeLa and E.coli BL21...
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