Direct-Blot™ HRP anti-Insulin Degrading Enzyme (IDE) Antibody, Insulin Degrading Enzyme (IDE), 9B12.225

Pricing & Availability

Clone: 9B12.225 (See other available formats)
Regulatory Status: RUO
Other Names: Abeta-degrading protease, Insulin protease, Insulinase, Abeta-Degrading Protease
Isotype: Mouse IgG1, κ
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Product Citations: publications

9B12-225_DBHRP_IDE_Antibody_052220 — Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of Direct-Blot™ HRP anti-Insulin Degrading Enzyme (IDE) antibody (clone 9B12.225) overnight at 4°C. Proteins were visualized by chemiluminescence detection. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.

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921205

25 µL

$136

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921206

100 µL

$340

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Description

Insulin degrading enzyme (IDE), also known as insulinase, insulin protease or insulysin, cleaves the peptide hormone insulin with consequences for insulin response and resistance. Since its discovery, IDE has been shown to degrade a variety of bioactive peptides. Recently the finding that IDE clears intracellular and extracellular amyloid products has spurred research into the link between IDE function and human neurodegenerative disease such as Alzheimer’s.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against purified human erythrocyte IDE
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 9B12.225 has been shown to detect a ~118 kD isoform in peripheral tissue and a second isoform at ~54 kD in brain tissue.
RRID: AB_2861113 (BioLegend Cat. No. 921205)
AB_2861113 (BioLegend Cat. No. 921206)

Antigen Details

Structure: IDE is a 1019 amino acid protein with a molecular weight of ~ 118 kD.
Distribution: Tissue distribution: Abundantly expressed in gastrointestinal tract, muscle and nervous system tissues.
Cellular distribution: Secreted, mitochondrion, peroxisome, nucleus, cytosol, and plasm membrane.
Function: IDE cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is also the enzyme responsible for degradation and clearance of amyloid beta in the brain.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Tang WJ. 2016. Trends Endocrinol Metab. 27(1):24.

Jha NK, et al. 2015. J Alzheimers Dis. 48(4):891.
Gene ID: 3416 View all products for this Gene ID
UniProt: View information about Insulin Degrading Enzyme on UniProt.org

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Western Blotting Protocol

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Other Formats

View All Insulin Degrading Enzyme (IDE) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Insulin Degrading Enzyme (IDE)	9B12.225	WB
Direct-Blot™ HRP anti-Insulin Degrading Enzyme (IDE)	9B12.225	WB

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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