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- Clone
- 8C5.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DSRED
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
10 µg of total protein extract from 293E cells transfected with pcDNA3.1-mCherry was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-mCherry (clone 8C5.5). Proteins were visualized using chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 677703 | 100 µL | $340 | ||||
mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins1,2. mCherry derives from proteins initially isolated from Cnidarians.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- mCherry.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Product Citations
-
- RRID
-
AB_2566703 (BioLegend Cat. No. 677703)
Antigen Details
- Structure
- 236 amino acids with a predicted molecular weight of approximately 27 kD.
- Function
- Fluorescent protein.
- Biology Area
- Cell Biology
- Antigen References
-
1. Shaner NC, et al. 2008. Nat. Methods 5:545.
2. Shaner NC, et al. 2005. Nat. Methods 2:905. - Gene ID
- NA
- UniProt
- View information about mCherry on UniProt.org
Other Formats
View All mCherry Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mCherry | 8C5.5 | WB,ICC,IP |
| Direct-Blot™ HRP anti-mCherry | 8C5.5 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mCherry
Total cell lysates (15 µg total protein) from mock-transfect... 
mCherry-transfected 293T cells were stained with purified an... -
Direct-Blot™ HRP anti-mCherry
10 µg of total protein extract from 293E cells transfected ...
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