- Clone
- Poly4053 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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405312 | 100 µg | $106 |
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with DyLight™ 649 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* DyLight™ 649 has a maximum absorption of 655nm and a maximum emission of 670nm (similar to Alexa Fluor® 647 and APC). - Application Notes
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This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
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Application References
(PubMed link indicates BioLegend citation) -
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
-
- RRID
-
AB_1575128 (BioLegend Cat. No. 405312)
Antigen Details
- Distribution
-
B cells
- Gene ID
- 16059 View all products for this Gene ID
- UniProt
- View information about IgG on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Goat anti-mouse IgG (minimal x-reactivity)
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Biotin Goat anti-mouse IgG (minimal x-reactivity)
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FITC Goat anti-mouse IgG (minimal x-reactivity)
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HRP Goat anti-mouse IgG (minimal x-reactivity)
-
PE Goat anti-mouse IgG (minimal x-reactivity)
-
Purified Goat anti-mouse IgG (minimal x-reactivity)
-
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity)
-
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
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PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity)
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DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity)
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DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity)
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Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity)
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APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
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Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity)
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Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity)
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Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity)
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Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity)
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Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity)
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Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity)
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PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity)
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PerCP Goat anti-mouse IgG (minimal x-reactivity)
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APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity)
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Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity)
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Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity)
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