Pricing & Availability
Technical data sheet
- Regulatory Status
- RUO
- Other Names
- Fibroblast interferon
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
449504 | 5 plates | $350 |
Description
Type I Interferons (IFN-α/β) are produced primarily in response to viral infection by natural IFN-producing cells (IPCs) as part of the host immune response. IFN-β binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. IFN-β is currently used clinically for treatment of tumors, infections and multiple sclerosis.
Product DetailsKit Contents
- Kit Contents
-
- Human IFN-β ELISA MAX™ Capture Antibody (200X)
- Human IFN-β ELISA MAX™ Detection Antibody (200X)
- Human IFN-β Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer B (5X)
- Blocking Buffer A (2X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.60 + 0.11 pg/mL
- Standard Range
- 2.3 - 150 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Epithelial cells, Fibroblasts
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Innate Immune Signaling
- Gene ID
- 3456 View all products for this Gene ID
- UniProt
- View information about IFN-beta on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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