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- Regulatory Status
- RUO
- Other Names
- Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng, NRGN
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 430004 | 5 plates | $365 | ||||
Neurogranin (NRGN) is the main post-synaptic protein kinase substrate that binds to calmodulin (CaM) in the absence of calcium. NRGN’s size (78 amino acids, 7.6 kD) allows it to pass through a damaged blood–brain barrier, making it a biomarker in chronic neurodegenerative diseases and potentially for acute brain injury. Measurement of serum NRGN levels could support the early diagnosis of central nervous infections.
Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease. NRGN function has been implicated in learning and memory. It is suggested that the NRGN is a direct target for thyroid hormone in the human brain and the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development, as well as in adult subjects.
Kit Contents
- Kit Contents
-
- Human Neurogranin Capture Antibody (200X)
- Human Neurogranin Detection Antibody (200X)
- Human Neurogranin Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Blocking Buffer A (2X)
- Assay Diluent B (5X)
- Assay Diluent D
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 3.46 ng/mL
- Standard Range
- 6.25 - 400 ng/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- NA
- UniProt
- View information about Neurogranin on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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