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- Regulatory Status
- RUO
- Other Names
- Nucleocapsid (N) protein, SARS-CoV-2 N Antigen, Nucleocapsid phosphoprotein, Nucleoprotein, SARS-CoV-2 Nucleocapsid Protein
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 448004 | 5 plates | $373 | ||||
The nucleocapsid protein is an integral component of SARS-CoV-2 virus, and it is highly conserved among the coronaviruses. The protein's main functions are interaction with viral RNA and the membrane protein during assembly, as well as nucleocapsid structure of the viral core. These functions increase the efficiency of viral transmission and assembly. In SARS and other viral infections, nucleocapsid protein levels in patient serum have been shown to be a descriptive marker of the disease progression. Nucleocapsid protein can be detected in patient serum early in disease onset, prior to antiviral IgG or IgM, and detection levels tend to stay high into the infection.
Product DetailsKit Contents
- Kit Contents
-
- SARS-CoV-2 Nucleocapsid Capture Antibody (200X)
- SARS-CoV-2 Nucleocapsid Detection Antibody (200X)
- SARS-CoV-2 Nucleocapsid Protein Standard
- Avidin-HRP (1000X)
- Substrate Solution D
- Coating Buffer B (5X)
- Assay Diluent B (5X)
- Blocking Buffer A (2X)
- Assay Buffer H
Product Details
- Verified Reactivity
- SARS-CoV-2
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 21.4 pg/mL
- Standard Range
- 125 - 8000 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Biology Area
- Immunology
- Gene ID
- NA
- UniProt
- View information about SARS-CoV-2 Nucleocapsid Protein on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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