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FITC anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
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Product Citations
publications
XMG1.2_FITC_IFNgamma_Antibody_ICFC_1_090712
  • XMG1.2_FITC_IFNgamma_Antibody_ICFC_1_090712
  • XMG1.2_FITC_IFNgamma_Antibody_ICFC_2_090712
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505805 25 µg $33
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505806 100 µg $99
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The IFN-γ antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  2. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  3. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  4. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  5. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  6. Chen N, et al. 2021. Front Immunol. 12:756423. PubMed
  7. Yamazaki S, et al. 2022. Mucosal Immunol. :. PubMed
  8. Schenkel JM, et al. 2021. Immunity. 54:2338. PubMed
  9. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  10. Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed
  11. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  12. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  13. Balood M, et al. 2022. Nature. 611:405. PubMed
  14. Xu H, et al. 2022. Aging Dis. 13:1875. PubMed
  15. Wang R, et al. 2023. iScience. 26:105954. PubMed
  16. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  17. Belk JA, et al. 2022. Cancer Cell. 40:768. PubMed
  18. Zhao L, et al. 2022. Oncogene. 41:4200. PubMed
  19. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  20. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  21. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  22. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  23. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  24. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  25. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  26. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  27. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  28. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  29. Ford K, et al. 2020. Cancer Res. 80:1846. PubMed
  30. Kim EH, et al. 2020. Elife. 9:00. PubMed
  31. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  32. Onodera A, et al. 2017. J Immunol. 10.4049/jimmunol.1602129. PubMed
  33. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  34. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  35. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  36. Magen A, et al. 2019. Cell Rep. 29:3019. PubMed
  37. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  38. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  39. Gao Y, et al. 2020. Nat Cell Biol. 1064:22. PubMed
  40. Weigelin B, et al. 2015. Proc Natl Acad Sci U S A. 112:7551. PubMed
  41. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  42. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  43. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  44. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  45. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  46. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  47. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  48. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  49. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  50. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  51. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  52. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  53. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  54. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  55. Larena M, et al. 2011. J Virol. 85:5446. PubMed
  56. Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
  57. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  58. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  59. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  60. Yang X, et al. 2019. Oncotarget. 10:4180. PubMed
  61. Alessandrini F, et al. 2019. Oncogene. 38:4467. PubMed
  62. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  63. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  64. Wei F, et al. 2014. Cancer Res. 74:5989. PubMed
  65. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  66. Khakhum N, et al. 2021. NPJ Vaccines. 6:72. PubMed
  67. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  68. Pu Y, et al. 2022. Transl Psychiatry. 12:223. PubMed
  69. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  70. Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
  71. Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
  72. Nika L, et al. 2019. Vaccines (Basel). 7. PubMed
  73. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  74. Li Y, et al. 2015. J Nutr Biochem. Available online 8 August 2015. PubMed
  75. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  76. Zhang L, et al. 2022. Front Immunol. 13:828263. PubMed
  77. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  78. Gudgeon N, et al. 2022. Cell Rep. 40:111193. PubMed
  79. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  80. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  81. Chung NH, et al. 2022. Vaccine. 40:574. PubMed
  82. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  83. Stutz MD, et al. 2021. Immunity. 54(8):1758-1771.e7. PubMed
  84. Tartey S, et al. 2021. Cancer Research. 81(9):2358-2372. PubMed
  85. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  86. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  87. Guo C, et al. 2017. Methods Mol Biol. 1709:345. PubMed
  88. Gao X, et al. 2010. Mol Immunol. 47:2397. PubMed
  89. Kedzierski L, et al. 2014. PLoS Pathog. 10:1004134. PubMed
  90. White C, et al. 2015. J Immunol. 194:697. PubMed
  91. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
  92. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  93. Sun C, et al. 2022. J Adv Res. 35:71. PubMed
  94. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  95. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  96. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  97. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  98. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  99. Pinget G, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.48. PubMed
  100. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  101. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  102. Montfort M, et al. 2004. J Immunol. 173:4084. PubMed
  103. Li Y, et al. 2021. Elife. 10:. PubMed
  104. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  105. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  106. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  107. Olivo Pimentel V, et al. 2021. J Immunother Cancer. 9:. PubMed
  108. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  109. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  110. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  111. Lebrun A, et al. 2015. J Immunol. 195: 4358 - 4368. PubMed
  112. Nakornpakdee Y, et al. 2018. Asian Pac J Allergy Immunol. 36:265. PubMed
  113. Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed
  114. M H, et al. 2016. Open Bio. 6: 150208. PubMed
  115. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  116. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  117. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  118. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  119. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  120. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  121. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  122. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  123. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  124. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  125. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  126. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  127. Zhu E, et al. 2014. J Immunol. 192:5599. PubMed
  128. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  129. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  130. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  131. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  132. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  133. Zeng J, et al. 2020. Int J Biol Sci. 1.939583333. PubMed
  134. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  135. Sartorius R, et al. 2018. Front Immunol. 1.413888889. PubMed
  136. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  137. Zamora-Chimal J, et al. 2017. Immunobiology. 222(2):454-462. PubMed
  138. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  139. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  140. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  141. Nakazawa S, et al. 2020. Sci Rep. 10:14559. PubMed
  142. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
  143. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  144. Angelou CC, et al. 2020. Frontiers in Immunology. 2.586805556. PubMed
  145. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_315399 (BioLegend Cat. No. 505805)
AB_315399 (BioLegend Cat. No. 505806)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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