FITC anti-rat CD25 Antibody anti-CD25

Pricing & Availability

Clone: OX-39 (See other available formats)
Regulatory Status: RUO
Other Names: IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
Isotype: Mouse IgG1, κ
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Product Citations: publications

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202103

100 µg

$153

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Description

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat T cell blasts from mixed lymphocyte reactions
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections¹, immunoprecipitation¹, weakly blocks IL-2 binding^{1, 2, 3}, and blocks IL-2 induced epithelial cell migration³.
RRID: AB_314001 (BioLegend Cat. No. 202103)

Antigen Details

Structure: Type I membrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
Function: Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand/Receptor: IL-2
Cell Type: B cells, Dendritic cells, Epithelial cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.
Gene ID: 16184 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD25 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-rat CD25	OX-39	FC
PE anti-rat CD25	OX-39	FC
Alexa Fluor® 488 anti-rat CD25	OX-39	FC
Biotin anti-rat CD25	OX-39	FC
APC anti-rat CD25	OX-39	FC
Brilliant Violet 421™ anti-rat CD25	OX-39	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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