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FITC Avidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Avidin-Fluorescein Isothiocyanate, Av-FITC
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Product Citations
publications
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
405101 100 µg $36
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405102 500 µg $118
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Description

Avidin binds to biotin with high affinity. Avidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical data sheet

Product Details

Formulation
10 mM HEPES, 0.5 M NaCl, pH 8.0, containing 0.08% sodium azide.
Preparation
Avidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
This solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Avidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Avidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Jeon OH, et al. 2007. Throb res. 119:609. PubMed
  2. Lin HY, et al. 2013. J. Virol. 87:9064. PubMed
Product Citations
  1. Jeon O, et al. 2007. Thromb Res. 119:609. PubMed
  2. Lin H, et al. 2013. J Virol. 87:9064. PubMed
  3. Quaranta M, et al. 2011. PLoS One. 6:e23442. PubMed
  4. Dalangood S, et al. 2020. Theranostics. 10:10078. PubMed

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Go To Top Version: 2    Revision Date: 01/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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