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Flash Phalloidin™ Red 594

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Regulatory Status
RUO
Other Names
Phalloidin
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Flash_Phalloidin_Red_594_1_040722
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with Flash Phalloidin™ Red 594 (5 µl of the stock solution in 200 µL of 5% FBS) for 20 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue).
  • Flash_Phalloidin_Red_594_1_040722
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with Flash Phalloidin™ Red 594 (5 µl of the stock solution in 200 µL of 5% FBS) for 20 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue).
  • Flash_Phalloidin_Red_594_2_040722
    C57 mouse frozen cerebellum tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the tissue was stained with Flash Phalloidin™ Red 594 (red, 25 µL of the stock solution in 1 mL of PBS) for 30 minutes at 4°C in the dark. The tissue was then counterstained with DAPI (blue). The image was captured by 20X objective.
See Flash Phalloidin™ Red 594 spectral data
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424203 300 units $347
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Description

Molecular Mass 1535.79 g/mol.  Flash Phalloidin™ Red 594 excites maximally at 590 nm and emits maximally at 611 nm. 

Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom.  This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which leads to cellular toxicity.  In cell imaging, this is a very useful probe for imaging and stabilizing filamentous F-actin in fixed and permeabilized cells, providing structural and volumetric context to the cell.  Phallotoxins are conjugated to a wide array of fluorophores to enable their use in multicolor microscopy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Preparation
Flash Phalloidin™ Red 594 is lyophilized. Reconstitute with 1.5mL of methanol to make a stock solution of 300 units.
Storage & Handling
Store Flash Phalloidin™ Red 594 at -20°C, protected from light.
Application

ICC - Quality tested
IHC-F - Verified
Recommended Usage

Reconstitute the Flash Phalloidin™ Red 594 with 1.5mL of 100% methanol to make 300 units. Then prepare a working concentration by diluting 1:20 - 1:100 of stock in 1X PBS. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
  2. Fix cultured cells with 1% - 4% paraformaldehyde (PFA) for 10 minutes at room temperature.
  3. Wash the cells two times with 1X PBS.
  4. Permeabilize the cells with 0.5% Triton X-100 for 10 minutes at room temperature or at 4°C.
  5. Wash the cells two times with 1X PBS.
  6. Block cells with 5% fetal bovine serum for 30 minutes at room temperature.
  7. Prepare the working solution by diluting Flash Phalloidin™ Red 594 1:20 - 1:100 in 1X PBS.
  8. Stain the cells with diluted solution for 20 minutes at room temperature in the dark protected from light.
  9. Mount the slides with an antifade mounting media and image the slides.

Antigen Details

Distribution

Cytoskeleton.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience
Gene ID
NA
Go To Top Version: 4    Revision Date: 10/26/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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