GMP Pacific Blue™ anti-human CD3 Antibody, CD3, UCHT1

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Clone: UCHT1 (See other available formats)
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

UCHT1_GMP_PB_CD3_Antibody_022322 — Typical results from human peripheral blood lymphocytes stained either with clone UCHT1 Pacific Blue™ used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP Pacific Blue™ spectral data

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260070

100 tests

$358

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical data sheet

Product Details

Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, and Western blotting⁹. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.

Beverly P, et al. 1981. Eur J Immunol. 11:329-34.

Lanier L, et al. 1986. J Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-human CD3	UCHT1	FC
Biotin anti-human CD3	UCHT1	FC
FITC anti-human CD3	UCHT1	FC
PE anti-human CD3	UCHT1	FC
PE/Cyanine5 anti-human CD3	UCHT1	FC
Purified anti-human CD3	UCHT1	FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3	UCHT1	FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3	UCHT1	FC,ICC,IHC-F
Pacific Blue™ anti-human CD3	UCHT1	FC
PE/Cyanine7 anti-human CD3	UCHT1	FC
Alexa Fluor® 700 anti-human CD3	UCHT1	FC
APC/Cyanine7 anti-human CD3	UCHT1	FC
PerCP anti-human CD3	UCHT1	FC
PerCP/Cyanine5.5 anti-human CD3	UCHT1	FC
Brilliant Violet 421™ anti-human CD3	UCHT1	FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3	UCHT1	FC
Ultra-LEAF™ Purified anti-human CD3	UCHT1	FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready)	UCHT1	FC,CyTOF®
Alexa Fluor® 594 anti-human CD3	UCHT1	ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
Brilliant Violet 510™ anti-human CD3	UCHT1	FC
Brilliant Violet 605™ anti-human CD3	UCHT1	FC
Brilliant Violet 711™ anti-human CD3	UCHT1	FC
Brilliant Violet 650™ anti-human CD3	UCHT1	FC
APC/Fire™ 750 anti-human CD3	UCHT1	FC
Pacific Blue™ anti-human CD3	UCHT1	FC
Brilliant Violet 785™ anti-human CD3	UCHT1	FC
PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
TotalSeq™-A0034 anti-human CD3	UCHT1	PG
TotalSeq™-B0034 anti-human CD3	UCHT1	PG
TotalSeq™-C0034 anti-human CD3	UCHT1	PG
PE anti-human CD3	UCHT1	FC
PE/Cyanine7 anti-human CD3	UCHT1	FC
KIRAVIA Blue 520™ anti-human CD3	UCHT1	FC
Spark Violet™ 538 anti-human CD3 Antibody	UCHT1	FC
TotalSeq™-D0034 anti-human CD3	UCHT1	PG
Spark Blue™ 574 anti-human CD3 Antibody	UCHT1	FC
GMP Pacific Blue™ anti-human CD3	UCHT1	FC
GMP PE anti-human CD3	UCHT1	FC
GMP PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
Spark Violet™ 423 anti-human CD3	UCHT1	FC
GMP PE/Cyanine7 anti-human CD3	UCHT1	FC
Spark Blue™ 515 anti-human CD3	UCHT1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/23/2022

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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