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GoInVivo™ Purified anti-mouse/rat IL-1β Antibody

Pricing & Availability
Clone
B122 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Armenian Hamster IgG
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503507 5 mg $770
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503508 25 mg $2365
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503509 50 mg $3575
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503510 100 mg $5335
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503511 500 mg $18425
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503512 1 g $28600
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli -expressed, recombinant mouse IL-1β
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.

Application References

(PubMed link indicates BioLegend citation)
  1. Hogquist K, et al. 1991. J. Immunol. 146:1534.
  2. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
  3. Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
  4. Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
  5. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
  6. Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
  7. Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
  8. Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed
RRID
AB_2888752 (BioLegend Cat. No. 503507)
AB_2888752 (BioLegend Cat. No. 503508)
AB_2888752 (BioLegend Cat. No. 503509)
AB_2888752 (BioLegend Cat. No. 503510)
AB_2888752 (BioLegend Cat. No. 503511)
AB_2888752 (BioLegend Cat. No. 503512)

Antigen Details

Structure
Cytokine; 17.5 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
16176 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-1β Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse / rat IL-1β B122 ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB
Biotin anti-mouse / rat IL-1β B122 ELISA
Ultra-LEAF™ Purified anti-mouse / rat IL-1β B122 ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB
GoInVivo™ Purified anti-mouse/rat IL-1β B122 ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB
Go To Top Version: 1    Revision Date: 10/08/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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