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HRP anti-β-Dystroglycan Antibody

Pricing & Availability
Clone
4F7 (See other available formats)
Regulatory Status
RUO
Other Names
Beta-dystroglycan, Beta-DG, DAG1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
4F7_HRP_beta-Dystroglycan_Antibody_1_041118
Western blot of HRP anti-β-Dystroglycan antibody (clone 4F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.5 µg/mL of clone 4F7 or mouse IgG overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. The antibody also detects a smaller band, slightly higher than the 25 kD molecular weight marker, which corresponds to a cleavage product of β-dystroglycan as previously reported.
  • 4F7_HRP_beta-Dystroglycan_Antibody_1_041118
    Western blot of HRP anti-β-Dystroglycan antibody (clone 4F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.5 µg/mL of clone 4F7 or mouse IgG overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. The antibody also detects a smaller band, slightly higher than the 25 kD molecular weight marker, which corresponds to a cleavage product of β-dystroglycan as previously reported.
  • 4F7_HRP_beta-Dystroglycan_Antibody_2_041118
    IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4F7_HRP_beta-Dystroglycan_Antibody_3_041118
    IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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849403 25 µg $129
 
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Description

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and utrophin scaffolds axin to the cytoskeleton. β-dystroglycan has also been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sumita K, et al. 2007. J. Neurochem. 100(1):154. (IP, WB)
RRID
AB_2734626 (BioLegend Cat. No. 849403)

Antigen Details

Structure
Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution

Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.

Cellular distribution: Nucleus, plasma membrane, and cytoskeleton.

Function
β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction
Interacts with dystrophin, utrophin and other cytoskeletal components such as axin.
Ligand/Receptor
Receptor for dystrophin and utrophin.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Antigen References

1. Murphy S and Ohlendieck K. 2015. Comput. Struct. Biotechnol. J. Nov 26;14:20-7.
2. Moore CJ and Winder SJ. 2012. Neuromuscul. Disord. Nov: 22(11): 959.

Gene ID
1605 View all products for this Gene ID
UniProt
View information about beta-Dystroglycan on UniProt.org
Go To Top Version: 1    Revision Date: 04/13/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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