HRP anti-DJ-1 PARK7 Antibody anti-DJ-1

Pricing & Availability

Clone: A16125E (See other available formats)
Regulatory Status: RUO
Other Names: Protein DJ-1, oncogene, DJ1, PARK7, Parkinson disease protein 7, Parkinsonism-Associated Deglycase, HEL-S-67p, epididymis secretory sperm binding protein Li 67p, GATD2, Protein Deglycase DJ-1
Isotype: Mouse IgG2b, κ
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Product Citations: publications

A16125E_HRP_DJ-1_Antibody_1_WB_050318 — Western blot of HRP anti-DJ-1 (PARK7) antibody (clone A16125E). Lane 1: Molecular weight marker; Lane 2: 20 µg of recombinant human DJ-1 (PARK7) protein (Cat. No. 843401); Lane 3: 20 µg of normal human brain lysate; Lane 4: 20 µg of Parkinson’s disease brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.

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851504

100 µg

$317

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Description

DJ-1 (PARK7) belongs to the peptidase C56 family of proteins and is expressed in almost all human cells and tissues. DJ-1 is a multi-functional protein associated with mitochondrial function, mitophagy, and male fertility. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease. Therefore, the protein has an important role in cell protection against oxidative stress and cell death. DJ-1 is also associated with prostate cancer, and specific mutations of the protein are linked to autosomal recessive, early onset Parkinson’s disease.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human DJ-1
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2734642 (BioLegend Cat. No. 851504)

Antigen Details

Structure: Human DJ-1 is a 189 amino acid protein with an apparent molecular weight of 20 kD. The protein consists of 7 β-strands and 7 α-helices, and exits as a dimer.
Distribution: Tissue distribution: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in brain and placenta.
Cellular distribution: Mitochondria, nucleus, cytosol, plasma membrane, and extracellular.
Function: DJ-1 functions as an oxidative stress sensor and redox-sensitive chaperone.
Interaction: PINK1, Parkin, EFCAB6/DJBP, OTUD7B, BBS1, HIPK1, CLCF1 and MTERF.
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: Mitochondrial Markers
Antigen References: Saito Y. 2014. J. Clin. Biochem. Nutr. 54(3):138-44. PubMed

Ariga H, et al. 2013. Oxid. Med. Cell Longev. 2013:683920. PubMed

Bonifati V, et al. 2003. Science (5604): 256–9. PubMed
Gene ID: 11315 View all products for this Gene ID
UniProt: View information about DJ-1 on UniProt.org

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Other Formats

View All DJ-1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-DJ-1 (PARK7)	A16125E	WB,Direct ELISA,IHC-P
HRP anti-DJ-1 (PARK7)	A16125E	WB,IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/04/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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