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ELISA MAX™ Standard Set Human IL-10

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-10 ELISA Kit
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Product Citations
publications
human il-10_122909
  • human il-10_122909
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430601 5 plates $265
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human IL-10 ELISA MAX™ Capture Antibody (200X)
  • Human IL-10 ELISA MAX™ Detection Antibody (200X)
  • Human IL-10 Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Jiang D,et al. 2008. Stem Cells. 26:2372. PubMed
  2. Takahash H, et al. 2014. PLoS One. 9:113722. PubMed
Product Citations
  1. Hasnat MA, et al. 2022. Immunol Cell Biol. 100:605. PubMed
  2. Fionda C, et al. 2022. Front Immunol. 13:1039120. PubMed
  3. Waqas FH, et al. 2022. Clin Transl Med. 12:e931. PubMed
  4. Singhal J, et al. 2022. Front Cell Infect Microbiol. 12:878136. PubMed
  5. Alhakeem SS, et al. 2018. J Immunol. 200:4180. PubMed
  6. Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
  7. Russell BJ, et al. 2022. Cell. 185:3263. PubMed
  8. Takahashi H, et al. 2014. PLoS One. 9:113722. PubMed
  9. Jiang D, et al. 2008. Stem Cells. 26:2372. PubMed
  10. Brandao-Rangel MAR, et al. 2021. Sci Rep. 11:17337. PubMed
  11. Croasdell A, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L888 - L901.. PubMed
  12. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  13. Xu D, et al. 2021. Front Oncol. 11:738607. PubMed
  14. Kumar P, et al. 2021. Front Immunol. 12:694865. PubMed
  15. Kim Y,et al. 2017. Adv Healthc Mater.. 10.1002/adhm.201700707. PubMed
  16. Nieto C, et al. 2018. Front Immunol. 9:31. PubMed
Standard Range
3.9-250 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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